Next-generation PCR buffer formulations for maximum PCR efficiency and reaction speed.Novel hot-start chemistry for improved specificity and sensitivity.Radiant™ qPCR Kits are available for intercalating-dye based assays (GREEN) and also hydrolysis probe assays (TaqMan® and molecular beacons). Radiant™ qPCR Kits are optimized for advanced multiplexing capability, delivering the same high efficiency, early Ct, and reaction speed even in complex multiplex assays. The proprietary buffer system allows for highly efficient amplification of GC-rich and AT-rich sequences in addition to significant improvements in PCR sensitivity in low-copy assays and PCR conditions conducive to ultra-fast amplification. Radiant™ qPCR Kits are engineered for robust real-time PCR with earlier quantification cycle values (Ct), industry-leading sensitivity (increased limit of detection) and speed (rapid extension rates). Fast run times, convenient packaging and timely delivery all make this family of reagents a regular best-seller.Ī high-performance portfolio of 2X ready-to-use qPCR mixes designed for best-in-class real-time PCR. “This all happened outside my fucking house,” one local said, surveying the debris, according to a clip posted by the Ukrainian journalist Illia Ponomarenko.We continually receive outstanding feedback from our Radiant PCR reagent users. Videos emerged on social media showing the aftermath of the combat with images of multiple destroyed armoured vehicles and several dead soldiers. Russian forces continued to slowly bear down on Kyiv, but forces were repelled in fighting in Bucha and Irpin, just to the north-west of the capital, after what appears to be a poorly coordinated attack by irregular forces. It said it had hit 1,067 Ukrainian military sites since the invasion began four days ago. Russia’s defence ministry claimed its “special operation” in Ukraine was a success. “Vasylkiv, Kyiv, Chernihiv, Sumy, Kharkiv and many other towns in Ukraine are living in conditions that were last experienced on our lands during the second world war,” he said. qR79LZOcJM- Illia Ponomarenko February 27, 2022

Part of my usual bike route in the Kyiv region. The brutal bombardment of a city of 1.4 million people showed Russia was deliberately shelling civilians in suburban areas, local people said.Īnd this is the road between Bucha and Irpin. It was too early to say on Sunday whether Moscow would mount a further attempt to capture Kharkiv. “Kharkiv will become for Russians a Ukrainian Stalingrad,” he wrote. Better to die with arms in your hands.”Īt 5pm machine-gun fire could be heard in several parts of the city, including in Poltavskyi Shliach Street, in the centre.Īnton Herashchenko, an adviser to Ukraine’s interior minister, who posted videos of the battle on his Telegram channel, said the Russian army had overreached itself. But if it comes to it I will take a fucking machine gun before I die like this and sit inside being shot, if they storm the basement.

People are fighting for their own homes, their families, their loved ones. The city would defeat occupying Russian forces, he predicted. People are sleeping in them because there isn’t enough space on the platforms.” The metro stopped working on Thursday afternoon and is now being used as a bomb shelter. It was his third night in a shelter, he said. One of the group was an electrician who had fixed the power supply, allowing people to charge their phones.

Volodymyrovich said he was hiding in a basement with 30 others, mostly women, children and elderly people. Ukrainians are still in control, volunteers are working, the mayor is in charge, and we are fighting back,” he said at 11am local time. “Diversionary Russian groups have been getting into Kharkiv. They passed Peremoha metro station, the final stop on the city’s green line, and headed towards the centre, he said, speaking from a basement shelter. Artem Volodymyrovich, a 31-year-old English teacher, said Russian units had advanced from the north.

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